Department of Biochemistry and Molecular Biology, J. Stefan Institute List of publications in 1995 in alphabetical order of the first authors Gregor Anderluh, Joze Pungercar, Borut Strukelj, Peter Macek, Franc Gubensek (1995) The coding region of the equinatoxin II gene lacks introns. Croat. Chem. Acta 68 (3) 533-542 (S-518) Joze Brzin, Marjetka Kidric (1995) Proteinases and their inhibitors in plants: Role in normal growth and in response to various stress conditions. Biotechnol. Gen. Engng. Rev. 13, 421-467 (S-536) Iztok Dolenc, Boris Turk, Galina Pungercic, Anka Ritonja, Vito Turk (1995) Oligomeric structure and substrate induced inhibition of human cathepsin C. J. Biol. Chem. 270 (37) 21626-21631 (S-516) Marko Dolinar, Darja Barlic Maganja, Vito Turk (1995) Expression of full-length human procathepsin L cDNA in Escherichia coli and refolding of the expression product. Biol. Chem. Hoppe-Seyler 376, 385-388 (S-507) Eva Ersdal-Badjou, Aiqin Lu, Xiaoming Peng, Veronique Picard, Pedram Zendehrouh, Boris Turk, Ingemar Bjork, Steven T. Olson, Susan C. Bock (1995) Elimination of glycosylation heterogeneity affecting heparin affinity of recombinant human antithrombin III by expression of a beta-like variant in baculovirus-infected insect cells. Biochem. J. 310, 323-330 Roman Hlodan, Roger H. Pain (1995) The folding and assembly pathway of tumour necrosis factor TNFalpha, a globular trimeric protein. Eur. J. Biochem. 231, 381-387 (S-511) Roman Jerala, Gordon S. Rule (1995) A 3D 1H, 15N and 13C NOESY correlating experiment. J. Magn. Reson. 108, 294-298 (S-517) Dusan Kordis, Franc Gubensek (1995) Horizontal SINE transfer between vertebrate classes. Nature Genetics 10, 131-132 (S-509) Janko Kos, Alojz Smid, Marta Krasovec, Branka Svetic, Brigita Lenarcic, Ivan Vrhovec, Janez Skrk, Vito Turk (1995) Lysosomal proteases cathepsins D, B, H, L and their inhibitors stefins A and B in head and neck cancer. Biol. Chem. Hoppe-Seyler 376, 401-405 (S-510) Igor Krizaj, E.G. Rowan, Franc Gubensek (1995) Ammodytoxin A acceptor in bovine brain synaptic membranes. Toxicon 33 (4), 437-449 (S-506) Robert Kuhelj, Marko Dolinar, Joze Pungercar, Vito Turk (1995) The preparation of catalytically active human cathepsin B from its precursor expressed in Escherichia coli in the form of inclusion bodies. Eur. J. Biochem. 229, 533-539 (S-504) Tamara T. Lah, Marguerite Hawley, Kenneth L. Rock, Alfred L. Goldberg (1995) Gamma-interferon causes a selective induction of the lysosomal proteases, cathepsins B and L, in macrophages. FEBS Lett. 363, 85-89 (S-500) Tamara T. Lah, Gloria Calaf, Endre Kalman, Balasahib G. Shinde, Jose Russo, David Jarosz, James Zabrecky, Robert Somers, Ierachimel Daskal (1995) Cathepsins D, B and L in breast carcinoma and in transformed human breast epithelial cells(HBEC). Biol. Chem. Hoppe-Seyler 376, 357- 363 (S-508) John R. Martin, C. Jeremy Craven, Roman Jerala, Louise Kroon-Zitko, Eva Zerovnik, Vito Turk, Jonathan P. Waltho (1995) The three-dimensional solution structure of human stefin A. J. Mol. Biol. 246, 331-343 (S-495) Roger Pain (1995) Overview of protein folding. In: Current protocols in protein science 6.4.1-6.4.7. John Wiley & Sons (S-544) Alenka Paris, Borut Strukelj, Joze Pungercar, Metka Renko, Iztok Dolenc, Vito Turk (1995) Molecular cloning and sequence analysis of human preprocathepsin C. FEBS Lett. 369, 326-330 (S-512) J.Z. Pedersen, B. Lomonte, R. Massoud, Franc Gubensek, J.M. Gutierrez, S. Rufini (1995) Autocatalytic acylation of phospholipase-like myotoxins. Biochemistry 34 (14) 4670-4675 (S-503) Maja Ravnikar, Borut Strukelj, Mateja Poljsak-Prijatelj, Irena Mavric, Pika Mesko, Joze Pungercar, Igor Kregar (1995) Immunolocalization of aspartic proteinase inhibitors in potato shoots induced by jasmonic acid in vitro. Acta Pharm. 45, 139-145 (S-515) Karl Ritsert, Robert Huber, Dusan Turk, Rudolf Ladenstein, Karen Schmidt-Baese, Adalbert Bacher (1995) Studies of the lumazine synthase/riboflavin synthase complex of Bacillus subtilis: Crystal structure analysis of reconstituted, icosahedral beta-subunit capsids with bound substrate analogue inhibitor at 2.4 A resolution. J. Mol. Biol. 253, 151-167 (S-523) Veronika Stoka, Maria Nycander, Brigita Lenarcic, Carlos Labriola, Juan Jose Cazzulo, Ingemar Bjork, Vito Turk (1995) Inhibition of cruzipain, the major cysteine proteinase of the protozoan parasite, Trypanosoma cruzi, by proteinase inhibitors of the cystatin superfamily. FEBS Lett. 370, 101-104 (S-514) Borut Strukelj, Joze Pungercar, Gregor Kopitar, Metka Renko, Brigita Lenarcic, Selma Berbic, Vito Turk (1995) Molecular cloning and identification of a novel porcine cathelin-like antibacterial peptide precursor. Biol. Chem. Hoppe-Seyler 376, 507-510 (S-513) Borut Strukelj, Maja Ravnikar, Pika Mesko, Mateja Poljsak- Prijatelj, Joze Pungercar, Gregor Kopitar, Igor Kregar, Vito Turk (1995) Molecular cloning and immunocytochemical localization of jasmonic acid inducible cathepsin D inhibitors from potato. In: Aspartic Proteinases: Structure, Function, Biology, and Biomedical Implications (Kenji Takahashi, ed.), Plenum Press, New York, pp. 293- 298 (S-520) Boris Turk, Anka Ritonja, Ingemar Bjork, Veronika Stoka, Iztok Dolenc, Vito Turk (1995) Identification of bovine stefin A, a novel protein inhibitor of cysteine proteinases. FEBS Lett. 360, 101-105 (S-496) Boris Turk, Joseph G. Bieth, Ingemar Bjork, Iztok Dolenc, Dusan Turk, Nina Cimerman, Janko Kos, Adrijana Colic, Veronika Stoka, Vito Turk (1995) Regulation of the activity of lysosomal cysteine proteinases by pH-induced inactivation and/or endogenous protein inhibitors, cystatins. Biol. Chem. Hope-Seyler 376, 225-230 (S-502) Boris Turk, Veronika Stoka, Ingemar Bjork, Christian Boudier, Gunnar Johansson, Iztok Dolenc, Adrijana Colic, Joseph G. Bieth, Vito Turk (1995) High-affinity binding of two molecules of cysteine proteinases to low-molecular- weight kininogen. Protein Science 4, 1874-1880 (S-519) Dusan Turk, Marjeta Podobnik, Tatjana Popovic, Nobuhiko Katunuma, Wolfram Bode, Robert Huber, Vito Turk (1995) Crystal structure of cathepsin B inhibited with CA030 at 2.0-A resolution: A basis for the design of specific epoxysuccinyl inhibitors. Biochemistry 34 (14) 4791-4797 (S-501) Vito Turk, Veronika Stoka, Boris Turk, Brigita Lenarcic (1995) Properties, structure and interaction of lysosomal cysteine proteinases with their protein inhibitors cystatins. In: Expression of tissue proteinases and regulation of protein degradation as related to meat quality (A. Ouali, D.I. Deymer, F.J.M. Smulders, eds.) ECCEAMST, pp. 199-212 (S-522) _________________________________________________________ List of publications in 1996 Benjamin Adams, Richard Fowler, Martin Hudson, Roger Pain (1996) The role of the C-terminal lysine in the hinge bending mechanism of yeast phosphoglycerate kinase. FEBS Lett. 385, 101-104 (S-526) Gregor Anderluh, Joze Pungercar, Borut Strukelj, Peter Macek, Franc Gubensek (1996) Cloning, sequencing, and expression of equinatoxin II. Biochem. Biophys. Res. Comm. 220, 437-442 (S-525) Tadeja Bevec, Veronika Stoka, Galina Pungercic, Iztok Dolenc, Vito Turk (1996) Major histocompatibility complex class II-associated p41 invariant chain fragment is a strong inhibitor of lysosomal cathepsin L. J. Exp. Med. 183, 1331-1338 (S-529) Marjan Budihna, Primoz Strojan, Lojze Smid, Janez Skrk, Ivan Vrhovec, Avgust Zupevc, Zvonimir Rudolf, Miha Zargi, Marta Krasovec, Branka Svetic, Natasa Kopitar-Jerala, Janko Kos (1996) Prognostic value of cathepsins B, H, L, D and their endogenous inhibitors stefins A and B in head and neck carcinoma. Biol. Chem. Hoppe-Seyler 377, 385-390 (S-539) Nina Cimerman, Marinka Drobnic Kosorok, Bruce D. Korant, Boris Turk, Vito Turk (1996) Characterization of cystatin C from bovine parotid glands: Cysteine proteinase inhibition and antiviral properties. Biol. Chem. Hoppe- Seyler 377, 19-23 (S-521) Iztok Dolenc, Boris Turk, Janko Kos, Vito Turk (1996) Interaction of human cathepsin C with chicken cystatin. FEBS Lett. 392, 277-280 (S-541) Roman Jerala, Paulo F.F. Almeida, Rodney L. Biltonen (1996) Simulation of the gel-fluid transition in a membrane composed of lipids with two connected acyl chains: Application of a dimer-move step. Biophys. J. 71, 609-615 (S-534) Roman Jerala, Paulo F.F. Almeida, Qiang Ye, Rodney L. Biltonen, Gordon S. Rule (1996) 1H, 15N and 13C resonance assignments and secondary structure of group II phospholipase A2 from Agkistrodon piscivorus piscivorus: Presence of an amino-terminal helix in solution. J. Biomolec. NMR 7, 107-120 (S-535) Gregor Kopitar, Marko Dolinar, Borut Strukelj, Joze Pungercar, Vito Turk (1996) Folding and activation of human procathepsin S from inclusion bodies produced in Escherichia coli. Eur. J. Biochem. 236, 558-562 (S-524) Dusan Kordis, Franc Gubensek (1996) Ammodytoxin C gene helps to elucidate the irregular structure of Crotalinae group II phospholipase A2 genes. Eur. J. Biochem. 240, 83- 90 (S-540) Janko Kos (1996) Lysosomal proteases and their inhibitors in malignant progression. In: Proceedings of the Croatian- Slovenian Meeting Molecular Oncology Today (Maja Osmak and Janez Skrk, eds.), Croatian League against Cancer, Zagreb, pp. 89-96 (S-530/1) Janko Kos, Borut Stabuc, Marta Krasevec, Ana Schweiger, Branka Svetic, Natasa Kopitar-Jerala, Janja Ocvirk, Nina Cimerman, Zvonimir Rudolf, Ivan Vrhovec (1996) Lysosomal proteases cathepsins B, H, L and their inhibitors stefin A and cystatin C in sera of patients with metastatic melanoma. In: Proceedings of the Croatian-Slovenian Meeting Molecular Oncology Today (Maja Osmak and Janez Skrk, eds.), Croatian League against Cancer, Zagreb, pp. 97-103 (S-530/2) Janko Kos (1996) Cathepsins & their inhibitors - new factors for prognosis in cancer. Eur. Clin. Lab., June 1996 (S-538) Igor Krizaj, Grazyna Faure, Franc Gubensek, Cassian Bon (1996) Re-examination of crotoxin-membrane interactions. Toxicon 34, 1003-1009 (S-546) Igor Krizaj, Avner Bdolah, Franc Gubensek, Petra Bencina, Joze Pungercar (1996) Protein and cDNA structures of an acidic phospholipase A2, the enzymatic part of an unususal, two- component toxin from Vipera palestinae, Biochem. Biophys. Res. Comm. 227, 374-379 (S-547) Tamara T. Lah, Gloria Calaf, Endre Kalman, Balasahib G. Shinde, Robert Somers, Sandino Estrada, Enrique Salero, Jose Russo, Ierachimel Daskal (1996) Cathepsins D, B, and L in transformed human breast epithelial cells. Breast Canc. Res. Treat. 39, 221233 (S-537) Panayotis Ledakis, William T. Tester, Nirit Rosenberg, David Romero-Fischmann, Ierachimel Daskal, Tamara T. Lah (1996) Cathepsins D, B, and L in malignant human lung tissue. Clinical Cancer Res. 2, 561-568 (S-551) Brigita Lenarcic, Igor Krizaj, Petra Zunec, Vito Turk (1996) Differences in specificity for the interactions of stefins A, B and D with cysteine proteinases. FEBS Lett. 395, 113-118 (S- 548) Adrijana Leonardi, Boris Turk, Vito Turk (1996) Inhibition of bovine cathepsins L and S by stefins and cystatins. Biol. Chem. Hoppe-Seyler 377, 319-321 (S-528) Djurdjica Novak Despot, Janko Kos, Gregor Sersa, Maja Cemazar, Bojan Mitrovic, Janez Skrk, Franc Gubensek (1996) Boronated monoclonal antibodies: A new tool in cancer treatment. In: Proceedings of the Croatian-Slovenian Meeting Molecular Oncology Today (Maja Osmak and Janez Skrk, eds.), Croatian League against Cancer, Zagreb, pp. 175-178 (S-530/4) Roger Pain (1996) Determining the CD spectrum of a protein. Current protocols in protein science 7.6.1-7.6.23. J. Wiley & Sons (S-545) Tatjana Popovic, Vida Puizdar, Anka Ritonja, Joze Brzin (1996) Simultaneous isolation of human kidney cathepsins B, H, L and C and their characterisation. J. Chromatogr. B 681, 251-262 (S-533) Anka Ritonja, Theresa H.T. Coetzer, Robert N. Pike, Clive Dennison (1996) The amino acid sequences, structure comparisons and inhibition kinetics of sheep cathepsin L and sheep stefin B. Comp. Biochem. Physiol. 114B, 193-198 (S-542) Paola Storici, Alessandro Tossi, Brigita Lenarcic, Domenico Romeo (1996) Purification and structural characterization of bovine cathelicidins, precursors af antimicrobial peptides. Eur. J. Biochem. 238, 769-776 (S-532) Primoz Strojan, Marjan Budihna, Janez Skrk, Lojze Smid, Janko Kos, Ivan Vrhovec (1996) Cathepsins B, H, L, D and their endogenous inhibitors stefins A and B in head and neck carcinoma. In: Proceedings of the Croatian-Slovenian Meeting Molecular Oncology Today (Maja Osmak and Janez Skrk, eds.), Croatian League against Cancer, Zagreb, pp. 105-111 (S-530/3) Primoz Strojan, Marjan Budihna, Lojze Smid, Janez Skrk, Janko Kos, Ivan Vrhovec, Miha Zargi, Avgust Zupevc, Zvonimir Rudolf (1996) The role of cathepsins and stefins as prognostic factors in patients with carcinoma in the head and neck region. Med. Razgl. 35 Suppl. 6, 139-144 (S-555) Boris Turk, Veronika Stoka, Vito Turk, Gunnar Johansson, Juan Jose Cazzulo, Ingemar Bjork (1996) High-molecular-weight kininogen binds two molecules of cysteine proteinases with different rate constants. FEBS Lett. 391, 109-112 (S-531) Dusan Turk, Marjeta Podobnik, Robert Kuhelj, Marko Dolinar, Vito Turk (1996) Crystal structures of human procathepsin B at 3.2 and 3.3 A resolution reveal an interaction motif between a papain-like cysteine protease and its propeptide. FEBS Lett. 384, 211-214 (S-527) Marc Vanhove, Xavier Raquet, Thimothy Palzkill, Roger H. Pain, Jean-Marie Frere (1996) The rate-limiting step in the folding of the cis-Pro167Thr mutant of TEM-1 beta-lactamase is the trans to cis isomerization of a non-prolinne peptide bond. Proteins: Struct. Funct. Genet. 25, 104-111 (S-543) Alois Zajc, Maria Joao Romao, Boris Turk, Robert Huber (1996) Crystallographic and fluorescence studies of ligand binding to N-carbamoylsarcosine amidohydrolase from Arthrobacter sp. J. Mol. Biol. 263, 269-283 (S-549) Last updated: April 18, 1997 (last reprint S-559).