RECOMBINANT DNA TECHNOLOGY
Faculty of Chemistry and Chemical Technology, University of Ljubljana

Basic information
:

Lectures: 60 h, winter semester in the 4th year; compulsory course for biochemistry students
Practical course: 60 h, summer semester in the 4th year; compulsory for biochemistry students in group A

Lecturer: Asist. Prof. Dr. Marko Dolinar

Please check the Slovenian Web page for further information!

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Topics
(each topic = 2 h)

A1    Introduction, basics of recombinant DNA technology, histroy, legislation
A2    Laboratory organisms; Safety in rec. DNA technology

B1    DNA: replication, transcription; protein biosynthesis (brief summary). Electrophoretic techniques.
B2    Enzymes in rec. DNA technology.

C1
   Vectors. Reporter genes. Cell transformations.
C2    cDNA synthesis, prepraration of libraries.

D1
   DNA synthesis in vitro, PCR, DNA sequencing.
D2    Hybridisations, probes labelling techniques. DNA-microarrays.

E1
   Expression systems - introduction.
E2    Ekspression in prokaryotes: vectors, fusions, optimisation of production; directed localization, solubility, stability, renaturation.

F1
   Expression in yeasts in filamentous fungi.
F2    Expression in cultured animal and human cells.

G1    Mutagenesis approaches; protein engineering.
G2    Phage display. Yeast 2-hybrid systems.

H1
   Forensic genetics / DNA fingerprinting.
H2    DNA-diagnostics. Use of DNA in systematics and ecology. Production of recombinant reagents.

I1
   Molecular biotechnology: application of recombinant organisms; biomass production.
I2    Recombinant bacteria in agronomy. Recomb. DNA in plant systems.

J1
  Plants in molecular biotechnology. Genetically modified food.
J2    Transgenic animals; knockout technology. Gene silencing with RNAi.

K1
  Stem cells. Cloning of mammals.
K2   Human Genome Project. Non-human genomes.

L1
   Genomics- Proteomics. Pharmacogenomics.
L2    Medical applications of recombinant DNA technology. Gene therapy.

M1  
Patenting.
M2   Ethics.

N1-2
   Novel approaches in recombinant DNA technology.

O1    
Q & A
O2    Written and oral examinations.
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Recommended text-book:
Bernard R. Glick, Jack J. Pasternak: Molecular Biotechnology, Principles and applications of recombinant DNA, 3rd edition, ASM Press, Washington, 2003 [ISBN: 1555812694]

Contact e-mail .
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Last update: October 30, 2003.