RECOMBINANT DNA TECHNOLOGY
Faculty of Chemistry and Chemical Technology, University of
Ljubljana
Basic information:
Lectures: 60 h, winter semester in the 4th year;
compulsory course for biochemistry students
Practical course: 60 h, summer semester in the 4th year;
compulsory for biochemistry students in group A
Lecturer: Asist. Prof. Dr. Marko Dolinar
Please check the Slovenian Web page
for further information!
____________________________________________________________________________________________________
Topics (each topic = 2 h)
A1 Introduction, basics of recombinant DNA
technology, histroy, legislation
A2 Laboratory organisms; Safety in rec. DNA
technology
B1 DNA: replication, transcription; protein
biosynthesis (brief summary). Electrophoretic techniques.
B2 Enzymes in rec. DNA technology.
C1 Vectors. Reporter genes. Cell transformations.
C2 cDNA synthesis, prepraration of libraries.
D1 DNA synthesis in vitro, PCR, DNA sequencing.
D2 Hybridisations, probes – labelling techniques.
DNA-microarrays.
E1 Expression systems - introduction.
E2 Ekspression in prokaryotes: vectors, fusions,
optimisation of production; directed localization, solubility,
stability, renaturation.
F1 Expression in yeasts in filamentous fungi.
F2 Expression in cultured animal and human cells.
G1 Mutagenesis – approaches; protein
engineering.
G2 Phage display. Yeast 2-hybrid systems.
H1 Forensic genetics / DNA
fingerprinting.
H2 DNA-diagnostics. Use of DNA in systematics and
ecology. Production
of recombinant reagents.
I1 Molecular biotechnology: application of recombinant
organisms; biomass production.
I2 Recombinant bacteria in agronomy. Recomb. DNA in
plant systems.
J1 Plants in molecular biotechnology. Genetically modified
food.
J2 Transgenic animals; knockout technology. Gene
silencing with RNAi.
K1 Stem cells. Cloning
of mammals.
K2 Human Genome Project. Non-human genomes.
L1 Genomics- Proteomics. Pharmacogenomics.
L2 Medical applications of recombinant DNA
technology. Gene therapy.
M1 Patenting.
M2 Ethics.
N1-2 Novel approaches in recombinant DNA technology.
O1 Q & A
O2 Written and oral examinations.
__
Recommended text-book:
Bernard R. Glick, Jack J. Pasternak: Molecular
Biotechnology, Principles and applications of recombinant
DNA, 3rd edition, ASM Press, Washington, 2003 [ISBN: 1555812694]
Contact e-mail
.
_________________________
Last update: October 30, 2003.